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KMID : 0525720210260040161
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Journal of Chitin and Chitosan
2021 Volume.26 No. 4 p.161 ~ p.165
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Bioconversion of Flavonoid Glycosides from Impatiens noli-tangere L.by Commercial Enzyme
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Oh Yu-Jin
Lee Dong-Ho
Jeong Hea-Seok
Lee Min-Sung
Kim Dong-Kap
Kim Jae-Woo
Han Kyung-Min
Kim Dae-Wook
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Abstract
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The food grade commercial enzyme Cytolase PCL5 from Aspergillus niger was used for bioconversion of luteolin from Impatiens noli-tangere L. extract (ILLE). The contents of cynaroside (luteolin 7-O-glucoside) in ILLE was 96.24 μg/g. The optimal conditions for production of luteolin from cynaroside using Cytolase PCL5 were pH 4.5, 60℃, 1,000 μg/g substrate, and 1 mg/mL enzyme. Under the optimum conditions, the Cytolase PCL5 converted cynaroside to luteolin with a molar yield of over 99% within 2 h. The IC50 value of DPPH radical scavenging activity and tyrosinase inhibitory effects of bioconverted ILLE were 72.8 and 98.71 μg/mL, respectively. The bioconverted ILLE exhibited 1.4 and 2.4 fold higher than I. noli-tangere L. These results indicated that the enzymatic bioconversion of ILLE can be used for developing a new natural functional material for cosmetic and food industry.
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KEYWORD
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Bioconversion, Cynaroside, Cytolase PCL5, Impatiens noli-tangere L., Luteolin
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